Modifying Enzymes
Carnabio Usa Inc PKCD(PRKCD)/5UG/FULL-LENGTH
PKC(PRKCD), Full-length, Wild type, Amino Acid 1-676, NP_006245.2, Expressed in Insect (sf21); 5microg
Agilent Technologies DNA POLYMERSAE HERCULASE II
II Fusion DNA Polymerase; Enhanced processivity; Inclusion of our exclusive ArchaeMaxx; Herculase;; 5X Herculase; II Reaction Buffer 1mL; Dimethyl Sulfoxide (DMSO) 1mL; 40 rxn; Storage: -20 deg.C
Aldevron Eureca-V Nuclease, 5mg
5mg dispense of Eureca-V Nuclease. CRISPR protein, Eureca-V™ Nuclease is the most recent addition to our catalog. It is based on the proprietary MAD7 nuclease developed by Inscripta. Aldevron and Inscripta have partnered to make this protein broadly available to the therapeutic, diagnostic, and agricultural research markets.Eureca-V is categorized as a Class 2, Type V CRISPR-Cas nuclease. It shares key features common to this family of CRISPR nucleases, including:
•Targets broad array of T-rich PAM domains (YTTV preferred, TTTN functions)
•Requires a single crRNA to cut target DNA sequence
•Staggered double-strand break at cut site creates 5’ overhangs
This product is subject to the following: Limited Use Label License
New England Biolabs, Inc. HiFi Taq DNA Ligase – 50 reactions
An optimized blend of a thermostable DNA Ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched high fidelity. The formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced in the improved reaction buffer and mismatch ligation is dramatically reduced. The improved formulation allows higher resolution discrimination between ligation donors and acceptors, enabling precise detection of SNPs and other allele variants. HiFi Taq DNA Ligase is active at elevated temperatures (37-75C). Please note that HiFi Taq DNA Ligase is intended for use in molecular diagnostics applications that depend on high fidelity nick ligation. It is not a substitute for T4 DNA ligase and is not suitable for cloning applications or adapter ligation/NGS library prep.
Apexbio Technology LLC Lambda Exonuclease(Synonyms: Lambda Exo, Lambda exonuclease enzyme, λ exonuclease, λ exonuclease enzyme), 10000U.
Lambda Exonuclease is a DNA-specific exonuclease enzyme that progressively degrades double-stranded DNA in a 5 3 direction preferentially initiating digestion at 5 -phosphorylated termini It displays minor activity toward single-stranded DNA and double-stranded DNA with non-phosphorylated ends but cannot start cleavage at internal nicks or gaps Lambda Exonuclease is commonly employed in molecular biology laboratories for the generation of single-stranded DNA templates DNA single-strand conformation polymorphism (SSCP) analysis selective degradation of linear plasmid forms while preserving supercoiled structures and rolling-circle amplification protocols
Bioworld Beta-Glactosidase, 1000 Unit(s)
Beta-Galactosidase Enzyme found in lac+ bacterial cells that catalyzes the cleavage of -linked terminal galactose-containing substratesLyophilized powderSource: E. coliConjugation gradeActivity (37°C):800-1000U/mg proteinUnit definition: One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH7.3 at 37°C, 410nm.Aggregation (HPLC): <3.0%Application: Used as an indicator enzyme conjugated to proteins such as antibodies.
Research Products International Corp Proteinase K, 100 Milligrams
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.
MOLECULAR DEPOT LLC Cholesterol Oxidase - 250 U
Cholesterol Oxidase (250 U) consists of a highly purified powdered Cholesterol Oxidase enzyme a flavoprotein involved in the first step of cholesterol catabolism The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide For in vitro research use only
Medchemexpress LLC α-amylase-IN-3 | 93944-58-2 | 99.5% | 267.24 g/mol | C15H9NO4 | 10 MG
α-Amylase-IN-3 is a small-molecule research reagent reported to inhibit α-amylase and acetylcholinesterase, and to exhibit antioxidant activity. It is supplied as a solid for laboratory research and is used in studies related to diabetes and oxidative stress-associated disease.
- Non-competitive α-amylase inhibitor with reported IC50 ≈ 18.04 μM.
- Acetylcholinesterase inhibitory activity with reported IC50s ≈ 21.04 μM and 22.2 μM.
- Demonstrates antioxidant activity in standard radical-scavenging assays.
- Supplied as a stable solid suitable for laboratory storage and handling.
- High reported purity (≈99.49% by HPLC).
- Molecular weight 267.24 g/mol; chemical formula C15H9NO4.
Aldevron SpyFi Cas9 Nuclease, 1mg
5mg dispense size of SpyFi Cas9 Nuclease. From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade through Fisher, or across all quality grades (Research Grade or GMP) directly through Aldevron.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
•Reduced off-target effects
•Ready to use for electroporation or transfection
•10 mg/mL formulation for ease of use
Carnabio Usa Inc MLK2(MAP3K10)/10UG/CATALYTIC
MLK2(MAP3K10), Catalytic domain, Wild type, Amino Acid 75-462, NP_002437.2, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. Tth Endonuclease IV – 500 units
Tth Endonuclease IV is a thermostable apurinic/apyrimidinic (AP) endonuclease from Thermus thermophilus.